Media

A ventral view of an L1 larvae expressing a tight junction::GFP (circles around P cells) and a GFP for fibrous organelles that connect muscles to the cuticle. For more details, see Bone et al 2016 Development.

Super-resolution image (SIM, DeltaVision OMX) of a serum-starved wound-edge mouse embryonic fibroblast that was fixed in paraformaldehyde 60 minutes after being stimulated with LPA. Blue: DNA (DAPI). Red: Actin (rhodamine-phalloidin).
Super-resolution image (SIM, DeltaVision OMX) of a serum-starved wound-edge mouse embryonic fibroblast that was fixed in paraformaldehyde 60 minutes after being stimulated with LPA. Blue: DNA (DAPI). Green LINC complexes (anti- nesprin-2G). Red: Actin (rhodamine-phalloidin).
Epifluorescence image (Nikon) of a serum-starved wound-edge mouse embryonic fibroblast that was fixed in paraformaldehyde 60 minutes after being stimulated with LPA. Blue: DNA (DAPI). Green: focal adhesions (anti-vinculin). Red: Actin (rhodamine-phalloidin).
Epifluorescence image (Nikon) of serum-starved wound-edge NIH3T3 fibroblasts that were fixed in methanol 120 minutes after being stimulated with LPA. Blue: DNA (DAPI). Green: microtubules (anti-tyrosinated microtubules). Red: centrosomes (anti-pericentrin).

Live epifluorescence (left) and phase contrast (right) imaging (Nikon) of a serum-starved wound-edge Lifeact-mCherry-expressing NIH3T3 fibroblast stimulated with LPA. Images were taken every 5 minutes after LPA-stimulation.

Spinning disk confocal (Olympus) image of full-length EGFP-tagged mouse SUN2 reconstituted in artificial nuclear membranes.
Pseudocolored epifluorescence image (Nikon) of abnormal nuclear envelope-associated lysine 48 (K48) poly-ubiquitination in tor1A/1B/2A/3A-knockout HeLa cells labeled by immunofluorescence.
A GFP marker is expressed in hypodermic syncytial nuclei. In this anc-1 mutant adult, the syncytial nuclei can be seen clustering. See Cain et al 2018 Current Biology for more details.
TEM image of an unc-84 mutant muscle cell nucleus in C. elegans. Notice the outer nuclear membrane is a large distance from the inner nuclear membrane. See Cain et al 2014, JCB for more details.

Live epifluorescence and phase contrast imaging (Nikon Biostation) of serum-starved wound-edge EGFP-histone-2B- and dsRED-centrin1-expressing NIH3T3 fibroblasts stimulated with 10% serum. Images were taken ever 30 minutes after serum-stimulation.

Live epifluorescence imaging (Nikon) of serum-starved wound-edge of EGFP-mini-nesprin-2G-expressing and nesprin-2G-depleted NIH3T3 fibroblasts stimulated with LPA. Images were taken every 5 minutes after LPA-stimulation.
A movie in real time of the ER marked with GFP in an anc-1 mutant. In this mutant background, the ER is not anchored and has severe positioning defects. For more details, see Hao et al 2020 bioRxiv.
A movie of EB1::GFP showing the plus ends of growing microtubules in C. elegans embryonic hypodermic cells.
A movie of nuclear migration in a C. elegans embryonic hyp7 precursor. The movie on the left marks two nucleoli. For more details, see Fridolfsson et al 2010, JCB
Live DIC and spinning disk confocal imaging (3I) of mCherry-H2B expressing Dictyostelium discoideum.
Live spinning disk confocal imaging (3I) of Lifeact-mCherry (Actin) and EGFP-tubulin (microtubules) expressing Dictyostelium discoideum.